CcpA - Mediated Catabolite Activation of the Bacillus subtilis ilv - leu Operon 1 and Its Negation by Either CodY - or TnrA - Mediated Negative Regulation
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چکیده
CcpA-Mediated Catabolite Activation of the Bacillus subtilis ilv-leu Operon 1 and Its Negation by Either CodYor TnrA-Mediated Negative Regulation 2 3 4 Yasutaro Fujita*, Takenori Satomura, Shigeo Tojo, and Kazutake Hirooka 5 6 7 Department of Biotechnology, Faculty of Life Science and Biotechnology, Fukuyama 8 University, 985 Sanzo, Higashimura-cho, Fukuyama-shi, Hiroshima 729-0292, Japan 9 10 11 Running title: Catabolite activation of B. subtilis ilv-leu 12 13 Address correspondence to Yasutaro Fujita, [email protected]. 14 15 Supplemental material for this article may be found at XXX. 16 17 Present addresses: T. Satomura: Department of Applied Chemistry and 18 Biotechnology, Graduate School of Engineering, University of Fukui, Fukui 910-8507, 19 Japan; S. Tojo: Department of Food Sciences and Biotechnology, Hiroshima Institute 20 of Technology, Hiroshima 731-5193, Japan 21 JB Accepts, published online ahead of print on 25 August 2014 J. Bacteriol. doi:10.1128/JB.02055-14 Copyright © 2014, American Society for Microbiology. All Rights Reserved.
منابع مشابه
CcpA-mediated catabolite activation of the Bacillus subtilis ilv-leu operon and its negation by either CodY- or TnrA-mediated negative regulation.
The Bacillus subtilis ilv-leu operon functions in the biosynthesis of branched-chain amino acids. It undergoes catabolite activation involving a promoter-proximal cre which is mediated by the complex of CcpA and P-Ser-HPr. This activation of ilv-leu expression is negatively regulated through CodY binding to a high-affinity site in the promoter region under amino acid-rich growth conditions, and...
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The Bacillus subtilis ilv-leu operon is involved in the synthesis of branched-chain amino acids (valine, isoleucine, and leucine). The two- to threefold repression of expression of the ilv-leu operon during logarithmic-phase growth under nitrogen-limited conditions, which was originally detected by a DNA microarray analysis to compare the transcriptomes from the wild-type and tnrA mutant strain...
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Bacillus subtilis ccpA mutant strains exhibit two distinct phenotypes: they are defective in catabolite repression, and their growth on minimal media is strongly impaired. This growth defect is largely due to a lack of expression of the gltAB operon. However, growth is impaired even in the presence of glutamate. Here, we demonstrate that the ccpA mutant strain needs methionine and the branched-...
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